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Western blot

Definition(s)

The separation of proteins on an electrophoretic gel for identification by immunological techniques

Definition from: GeneReviewsThis link leads to a site outside Genetics Home Reference. from the University of Washington and the National Center for Biotechnology Information

Western blotting is a laboratory technique used to detect a specific protein in a blood or tissue sample. The method involves using gel electrophoresis to separate the sample's proteins. The separated proteins are transferred out of the gel to the surface of a membrane. The membrane is exposed to an antibody specific to the target protein. Binding of the antibody is detected using a radioactive or chemical tag. A western blot is sometimes used to diagnose disease.

Definition from: Talking Glossary of Genetic TermsThis link leads to a site outside Genetics Home Reference. from the National Human Genome Research Institute

See also Understanding Medical Terminology.

 
Published: August 24, 2015