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Northern blot

Definition(s)

The separation of sequences or fragments of RNA, partially digested by endonucleases, on an electrophoretic gel

Definition from: GeneReviewsThis link leads to a site outside Genetics Home Reference. from the University of Washington and the National Center for Biotechnology Information

A blot consisting of a sheet of cellulose nitrate or nylon that contains spots of RNA for identification by a suitable molecular probe.

Definition from: Merriam-Webster's Medical DictionaryThis link leads to a site outside Genetics Home Reference. by Merriam-Webster Inc.

Northern blot is a laboratory technique used to detect a specific RNA sequence in a blood or tissue sample. The sample RNA molecules are separated by size using gel electrophoresis. The RNA fragments are transferred out of the gel to the surface of a membrane. The membrane is exposed to a DNA probe labeled with a radioactive or chemical tag. If the probe binds to the membrane, then the complementary RNA sequence is present in the sample.

Definition from: Talking Glossary of Genetic TermsThis link leads to a site outside Genetics Home Reference. from the National Human Genome Research Institute

See also Understanding Medical Terminology.

 
Published: July 27, 2015