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Isoelectric focusing

Definition(s)

Method of mutation detection by which proteins are separated according to the pH at which their net charge is zero (isoelectric point); often used in conjunction with a western blot to allow identification of wild-type versus mutant protein products. A DNA sequence alteration resulting in an amino acid substitution can change the isoelectric point of a protein.

Definition from: GeneReviewsThis link leads to a site outside Genetics Home Reference. from the University of Washington and the National Center for Biotechnology Information

See also Understanding Medical Terminology.

 
Published: May 20, 2013