Skip Navigation
Genetics Home Reference: your guide to understanding genetic conditions About   Site Map   Contact Us
Home A service of the U.S. National Library of Medicine®
0-9 | A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q-R | S | T | U | V | W | X | Y-Z

Isoelectric focusing


Method of mutation detection by which proteins are separated according to the pH at which their net charge is zero (isoelectric point); often used in conjunction with a western blot to allow identification of wild-type versus mutant protein products. A DNA sequence alteration resulting in an amino acid substitution can change the isoelectric point of a protein.

Definition from: GeneReviewsThis link leads to a site outside Genetics Home Reference. from the University of Washington and the National Center for Biotechnology Information

See also Understanding Medical Terminology.

Published: February 1, 2016